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目的研究云南松松塔乙醇提取物(YN-DEF)体外对人肝癌细胞的作用。方法采用四甲基偶氮唑盐比色法(MTT法),观察云南松提取物对肝癌细胞(HEPG2、Huh-7)增殖的影响及对正常肝细胞(L-02)的作用。结果与阴性对照组比较,在浓度1000μg/ml,72h时,YN-DEF对HEPG2、Huh-7的抑制率分别为72.55%、60.66%,且抑制作用均呈浓度依赖性;正常肝细胞(L-02)与不同浓度为YN-DEF共同孵育72h后,高浓度下(1000μg/ml)的存活率为73.51%。结论云南松松塔乙醇提取物(YN-DEF)具有抑制肝癌细胞增殖的作用,高浓度下对正常肝细胞毒性小。
Objective To study the effect of pine nut tower ethanol extract (YN-DEF) on human hepatoma cells in vitro. Methods MTT method was used to observe the effect of extract of Yunnan pine on the proliferation of hepatocellular carcinoma cells (HEPG2 and Huh-7) and the effect on normal liver cells (L-02). Results Compared with the negative control group, the inhibitory rates of YN-DEF on HEPG2 and Huh-7 were 72.55% and 60.66% at the concentration of 1000μg / ml and 72h, respectively, and the inhibitory effects were in a concentration-dependent manner. -02) were incubated with different concentrations of YN-DEF for 72h, the survival rate of high concentration (1000μg / ml) was 73.51%. Conclusion The ethanol extract of Pinus yunnanensis (YN-DEF) has the effect of inhibiting the proliferation of hepatocellular carcinoma cells, and its toxicity to normal hepatocytes is low under high concentration.