论文部分内容阅读
[目的]评价重楼皂甙Ⅰ对肺腺癌细胞株PC9增殖和凋亡的影响。[方法]以体外培养的肺腺癌细胞株PC9为研究对象,MTT法检测重楼皂甙Ⅰ对PC9细胞增殖的抑制作用,流式细胞仪检测重楼皂甙Ⅰ对PC9细胞周期的影响,Annexin-V-FITC/PI双染法检测重楼皂甙Ⅰ对PC9细胞凋亡的影响,Western blot法检测重楼皂甙Ⅰ对PC9细胞Bcl-2、Bax、caspase-3蛋白表达的影响。[结果]不同浓度重楼皂甙Ⅰ能有效抑制PC9细胞的增殖,且呈时间浓度依赖性(P<0.01)。2.5μg/ml重楼皂甙Ⅰ作用PC9细胞12h、24h、48h后,出现G2/M期阻滞。2.5μg/ml重楼皂甙Ⅰ作用PC9细胞24h、48h后,细胞凋亡率明显增加,与对照组相比,具有统计学差异(P<0.01)。2.5μg/ml重楼皂甙Ⅰ作用PC9细胞48h后,Bcl-2蛋白表达降低、Bax及caspase-3蛋白表达增加,与对照组相比,亦具有统计学差异(P<0.01)。[结论]重楼皂甙Ⅰ能抑制PC9细胞的体外增殖,且抑制作用表现出时效和量效关系,其机制可能与G2/M期阻滞、促进细胞凋亡、降低Bcl-2蛋白表达、增加Bax及caspase-3蛋白表达有关。
[Objective] To evaluate the effect of resplenin Ⅰ on the proliferation and apoptosis of lung adenocarcinoma cell line PC9. [Method] The lung adenocarcinoma cell line PC9 was cultured in vitro. MTT assay was used to detect the inhibitory effect of resplenin Ⅰ on the proliferation of PC9 cells. Flow cytometry was used to detect the effect of resplenin Ⅰ on the cell cycle of PC9 cells. Annexin- The effect of resplenin Ⅰ on the apoptosis of PC9 cells was detected by V-FITC / PI double staining method. The effect of resplenin Ⅰ on the protein expression of Bcl-2, Bax and caspase-3 in PC9 cells was detected by Western blot. [Result] Resalin Ⅰ with different concentrations could effectively inhibit the proliferation of PC9 cells in a concentration - dependent manner (P <0.01). 2.5μg / ml resplenarch Ⅰ PC9 cells 12h, 24h, 48h, there G2 / M phase arrest. The apoptosis rate of PC9 cells treated with 2.5μg / ml polysaccharide Ⅰ for 24h or 48h was significantly higher than that of the control group (P <0.01). After treated with 2.5μg / ml resveratrol Ⅰ for 48h, the expression of Bcl-2 protein and Bax and caspase-3 protein increased in PC9 cells, which was also significantly different from the control group (P <0.01). [Conclusion] Polygonum saponin Ⅰ can inhibit the proliferation of PC9 cells in vitro, and its inhibitory effect shows the relationship of aging and dose-dependent. The mechanism may be related to the arrest of G2 / M phase, the promotion of apoptosis and the decrease of Bcl-2 protein expression Bax and caspase-3 protein expression.