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建立了用肽质量指纹谱和数据库检索方法鉴定凝胶电泳分离蛋白质的方法。用标准蛋白质对胶上蛋白质原位酶切制备肽谱的方法进行了讨论。分析了实际细胞蛋白质样品 ,获得了双向电泳分离的人肺癌细胞蛋白质谱中三个蛋白质点的肽指纹谱 ,并通过数据库检索分别鉴定为甘油醛 3 磷酸脱氢酶 2、遍在蛋白羧基末端水解同工酶和丙糖磷酸异构酶。
A method for the identification of proteins by gel electrophoresis using peptide mass fingerprinting and database search was established. The method of in situ digestion of the peptide on the gel protein with a standard protein is discussed. Peptide fingerprinting of three protein spots in the protein spectrum of human lung cancer cells isolated by two-dimensional electrophoresis was analyzed by real-life cell protein samples and identified as glyceraldehyde 3-phosphate dehydrogenase 2, carboxyterminal hydrolysis of ubiquitin Isozymes and triose phosphate isomerase.