论文部分内容阅读
目的探讨乙型肝炎病毒(HBV)阳性孕妇孕期足三里穴位注射乙型肝炎免疫球蛋白(HBIG)预防HBV宫内感染的效果。方法2001年11月至2003年10月烟台毓璜顶医院将190例乙型肝炎表面抗原(HB-sAg)阳性孕妇随机分为两组足三里穴位注射组92例,于孕20、24、28、32、36周足三里穴位注射HBIG共5次,每次200IU。肌肉注射组98例,同剂量、同时间应用HBIG。采用酶联免疫法,检测用药前后孕妇血清及新生儿脐血清HBsAg、HBeAg及HBsAb,采用核酸扩增(PCR)荧光定量检测HBV-DNA含量。结果92例足三里穴位注射者,新生儿HBV宫内感染8例,宫内感染率为8.7%,低于肌肉注射组(P<0.01)。穴位注射组新生儿脐血清HBsAb检出率显著高于肌肉注射组(P<0.05)。穴位注射组临产前血清中HBV-DNA显著低于肌肉注射组(P<0.05)。结论通过孕妇足三里穴位注射HBIG,可更有效地减少HBV的宫内感染,未发现任何副反应。
Objective To investigate the effect of hepatitis B virus immunization (HBIG) on HBV intrauterine infection in HBV-positive pregnant women during pregnancy. Methods From November 2001 to October 2003, 190 pregnant women with hepatitis B surface antigen (HBsAg) were randomly divided into two groups: 92 cases in Zusanli acupoint injection group at Yu 20, 24, 28, 32 , 36 weeks Zusanli points HBIG injection a total of 5 times, each 200IU. Intramuscular injection group 98 cases, with the same dose of HBIG. Serum and neonatal umbilical serum HBsAg, HBeAg and HBsAb were detected by enzyme-linked immunosorbent assay (ELISA) before and after treatment. HBV-DNA content was detected by fluorescence quantitative PCR. Results Eighty cases of neonatal HBV intrauterine infection were found in 92 cases of Zusanli acupoint injection. The intrauterine infection rate was 8.7%, which was lower than that of intramuscular injection group (P <0.01). The detection rate of neonatal umbilical serum HBsAb in acupoint injection group was significantly higher than that in intramuscular injection group (P <0.05). HBV-DNA in pre-delivery serum of acupoint injection group was significantly lower than that of intramuscular injection group (P <0.05). Conclusion Injecting HBIG through the points of pregnant women at Zusanli point can reduce HBV intrauterine infection more effectively and no side effects are found.