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[Objective]To explore the PCR-DGGE reaction system on rhizosphere soil microorganism for Panax ginseng C.A.Mey.[Method] Some important factors for PCR-DGGE amplification system including the concentration of DNA template,Mg2+,dNTPs and primers were optimized with 1-year-old P.ginseng soil as materials.[Result]An optimal system(25μl)was obtained as 0.8μg/μl DNA template,0.2 mmol/L dNTPs, 0.4μmol/L primer,and 1.5 mmol/L Mg2+.[Conclusion]The method is simple and fast,which can lay basis for further analyzing the genetic diversity of rhizosphere soil microorganism for P.ginseng.
[Objective] To explore the PCR-DGGE reaction system on rhizosphere soil microorganism for Panax ginseng CAMey. [Method] Some important factors for PCR-DGGE amplification system including the concentration of DNA template, Mg2 +, dNTPs and primers were optimized with 1- The result was as follows: 0.8 μg / μl DNA template, 0.2 mmol / L dNTPs, 0.4 μmol / L primer, and 1.5 mmol / L Mg2 +. ] The method is simple and fast, which can lay basis for further analyzing the genetic diversity of rhizosphere soil microorganism for P.ginseng.