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目的:建立通脱木组织培养与种苗快速繁殖技术体系。方法:试验以通脱木幼根、幼茎、幼叶和幼芽为外植体,以MS为基本培养基,利用不同的植物激素配比分别进行愈伤组织与丛芽的诱导、丛芽增殖与壮苗、生根诱导等,从中筛选出适宜的培养方案。结果:以培养基MS+NAA 3.0 mg/L+6-BA 2.0 mg/L诱导幼叶的出愈率较高,效果最好;以培养基MS+NAA 0.05 mg/L+6-BA 1.5 mg/L从愈伤组织诱导产生丛芽,诱导率最高,增殖系数为3.17倍;以培养基MS+NAA 0.05 mg/L+6-BA 1.5 mg/L+GA30.1 mg/L可促进丛芽增殖和壮苗;以培养基1/2MS+NAA 0.3 mg/L诱导生根,生根率达到90%以上。结论:建立了通脱木种苗快速繁殖技术体系,为通脱木种质资源保存和规模化生产奠定基础。
Objective: To establish a technical system of tissue culture and seedling rapid propagation. Methods: The experiment was carried out by using the seedlings of young seedlings, young stems, young leaves and young shoots as explants and MS as the basic medium. The callus and the buds were induced by using different plant hormones. Proliferation and strong seedlings, rooting induction, etc., from which screening of appropriate training programs. Results: The highest callus induction rate of young leaves was obtained with medium supplemented with MS + NAA 3.0 mg / L and 6-BA 2.0 mg / L. Medium MS + NAA 0.05 mg / L + 6-BA 1.5 mg / L from the callus induction buds, the highest induction rate of 3.17 times the proliferation; medium MS + NAA 0.05 mg / L + 6-BA 1.5 mg / L + GA30.1 mg / L can promote the bud Proliferation and seedling growth were observed. The rooting rate was above 90% with medium 1 / 2MS + NAA 0.3 mg / L. Conclusion: The rapid propagation technology system of seedlings is established, which lays the foundation for preserving and large-scale production of germplasm resources.