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用敏感细胞杀伤试验、ＭＴＴ比色法检测了３７例无先兆偏头痛患者和３５例正常健康人血浆ＴＮＦ的水平，结果发现偏头痛组ＴＮＦ水平为４９．１２±２１．２７μ／ｍｌ，高于正常对照组的４．８７±２．０１μ／ｍｌ（Ｐ＜０．００１），发作期高于缓解期（Ｐ＜０．０５）。认为ＴＮＦ在偏头痛的发病中起重要作用。
Sensitized cell killing test, MTT colorimetric detection of 37 patients without aura migraine and 35 normal healthy plasma TNF levels found that migraine group TNF level was 49.12 ± 21.27μ / ml, higher than The normal control group was 4.87 ± 2.01μ / ml (P <0.001), and the onset time was higher than that of the remission period (P <0.05). TNF is considered to play an important role in the development of migraine.