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目的:基于顶空气相色谱-质谱(HSGC-MS)技术,建立了分析不同产地来源的苍耳草和苍耳子中挥发性成分的方法。方法:采用HSGC-MS技术对苍耳草和苍耳子20批样品进行测定,通过NIST05标准质谱检索库对主要化合物进行鉴定,数据处理用SIMCA-P 11.5软件进行主成分分析。结果:主成分得分图显示,苍耳草及苍耳子样品间的差异得到明显区分,通过载荷图和T检验筛选出差异显著的11种标志物丙醛(Propanal,t R/min1.64)、二甲基硫醚(Dimethyl sulfide,t R/min 1.73)、2-甲基丙醛(Propanal,2-methyl-,t R/min 1.83)、正丁醛(Butanal,t R/min 1.95)、2,3-丁二酮(2,3-Butanedione,t R/min 2.07)、2-甲基丁醛(Butanal,2-methyl-,t R/min 2.48)、丙二酸(Propanedioic acid,t R/min 2.74)、1-氯戊烷(Pentane,1-chloro-,t R/min 3.62)、己醛(Hexanal,t R/min 4.62)、庚醛(Heptanal,t R/min 7.97)、a-蒎烯(1R.alpha-pinene,t R/min 9.53)。结论:从苍耳草与苍耳子挥发性成分差异的角度,可为揭示二者药性、药效差异的物质基础提供依据。
OBJECTIVE: To establish a method for the analysis of the volatile components in the radix paeoniaefolia and Xanthium sibiricum, based on the headspace gas chromatography-mass spectrometry (HSGC-MS) technique. Methods: Twenty batches of samples of Xanthium sanguineus and Xanthium sibiricum were tested by HSGC-MS. The main compounds were identified by NIST05 standard mass spectrometry library. The data were analyzed by SIMCA-P 11.5 software. Results: The score of the principal components showed that the differences between the samples of Xanthium sanguinea and Xanthium sibiricum were clearly distinguished. Propanal (tR / min1.64), a significant difference among the 11 markers, was screened out by load map and T test. , Dimethyl sulfide (t R / min 1.73), Propanal 2-methyl-, t R / min 1.83, Butanal (t R / min 1.95) , 2,3-Butanedione (tR / min 2.07), Butanal (2-methyl-, tR / min 2.48), Propanedioic acid tR / min 2.74), 1-chloropentane (Pentane, 1-chloro-, tR / min 3.62), hexanal (tR / min 4.62), heptanal (tR / min 7.97) , 1-pinene (tR / min 9.53). Conclusion: From the perspective of the differences in the volatile constituents of Xanthium sanguineus and Xanthium sibiricum, this study may provide the basis for revealing the material basis of their differences in pharmacodynamics and pharmacodynamics.