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目的简化测定胶原蛋白粉中呋喃类代谢物残留的实验过程,缩短实验的检测时间。方法在GB/T21311-2007的基础上,对其进行方法改进,建立高效液相色谱-串联质谱法(HPLC-MS/MS)测定胶原蛋白粉中呋喃西林(SEM)、呋喃妥因(AHD)、呋喃唑酮(AOZ)、呋喃它酮(AMOZ)等4种呋喃代谢物的快速检测方法。采用55℃衍生化,对衍生品按照离心、调p H值、离心的步骤进行处理,使得调节p H值更容易,同时避免传统试验方法中使用分液漏斗时样品乳化成胶状体而不能达到液液分离的目的的现象。结果实验结果表明,本方法与《GB/T21311-2007动物源性食品中硝基呋喃类药物代谢物残留量检测方法高效液相色谱串联质谱法》相比,结果并无很大的差异,但是大大缩短了实验的检测时间、简化了实验过程。结论本实验方法更加适合大批量样品的试验检测。
OBJECTIVE To simplify the experimental procedure for the determination of furan metabolites in collagen powder and to shorten the test time. Methods The method was improved on the basis of GB / T21311-2007 and established by HPLC-MS / MS for the determination of nitrofurazone (SEM), nitrofurantoin (AHD) and furazolidone AOZ), furan ketone (AMOZ) and other four kinds of furan metabolites rapid detection method. Derivatization with 55 ° C allows the derivative to be processed by centrifugation, pH adjustment, and centrifugation to make it easier to adjust the p H value while avoiding emulsification of the sample into a jelly-like body when using a separatory funnel in conventional test methods Liquid-liquid separation to achieve the purpose of the phenomenon. Results The experimental results showed that there was no significant difference between this method and “GB / T21311-2007 Detection method of residual amount of metabolites of nitrofuran in animal-derived food by high performance liquid chromatography-tandem mass spectrometry” Greatly reducing the test time of the experiment, simplifying the experiment process. Conclusion The experimental method is more suitable for testing large quantities of samples.