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为了鉴定脱氢表雄酮抗体的特异性及使用效价，观察脱氢表雄酮在人早期胎盘绒毛的细胞定位。方法：免疫组织化学ＡＢＣ法。结果：脱氢表雄酮抗体稀释度为１：１０００－１：２０００时，胎盘切片仍显示较强的脱氢表雄酮免疫反应阳性，抗体经过量的脱氢表雄酮一ＢＳＡ偶联复合物吸收后，再进行孵育时，胎盘切片呈阴性反应，提示该脱氢表雄酮抗体有较高的使用效价及特异性。人胎盘绒毛两层滋养层细胞和基质细胞均呈脱氢表雄酮免疫反应性，阳性反应物质主要分布在胞质，偶尔也在胞核内，提示人胎盘绒毛以上三种细胞可能能合成脱氢表雄酮，脱氢表雄酮对胎盘发育及妊娠维持可能有重要调节功能。
In order to identify the specificity of dehydroepiandrosterone antibody and the use of titers, we observed the cellular localization of dehydroepiandrosterone in early human placenta. Methods: Immunohistochemical ABC method. Results: When DHEA antibody dilution was 1: 1000-1: 2000, the placental sections still showed strong DHEA immunoreactivity, and the antibody was treated with dehydroepiandrosterone-BSA conjugate Absorption, and then incubated, the placental section was negative, suggesting that the dehydroepiandrosterone antibody with high titer and specificity. Human placental trophoblast cells and stromal cells were two dehydroepiandrosterone immunoreactivity, positive reactive substances are mainly distributed in the cytoplasm, and occasionally in the nucleus, suggesting that human placental cells may be synthesized above three kinds of hair cells DHEA and DHEA may play an important regulatory role in placental development and pregnancy maintenance.