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目的:探讨三七总皂苷(Panax notoginseng saponins,PNS)对神经胶质瘤U251和U87细胞增殖、迁移和凋亡的影响及其作用机制。方法:将U251和U87细胞各分为两组:对照(control)组和三七总皂苷(PNS)组。CCK-8检测细胞增殖水平;划痕实验检测细胞迁移能力;流式细胞仪检测细胞凋亡水平;Western Blot检测Caspase-3、Bax、Bcl-2、p-AKT、AKT、p-mTOR和mTOR的蛋白表达水平。结果:U251和U87细胞中的检测结果一致。与control组相比,PNS组的细胞增殖能力和迁移能力均显著下降(P<0.05);细胞凋亡率显著升高(P<0.05);Bcl-2的蛋白表达水平显著下调(P<0.05),而Bax和Caspase-3的表达水平显著上调(P<0.05);p-Akt和p-mTOR的蛋白表达水平下降,且差异具有显著性(P<0.05)。结论:PNS能够抑制U251和U87细胞的增殖和迁移,促进细胞凋亡,这可能与PI3K-Akt-mTOR信号通路的抑制有关。
Objective: To investigate the effects of panax notoginseng saponins (PNS) on proliferation, migration and apoptosis of gliomas U251 and U87 cells and its mechanism. Methods: U251 and U87 cells were divided into two groups: control group and PNS group. CCK-8 was used to detect the cell proliferation. Scratch assay was used to detect the migration ability of cells. Flow cytometry was used to detect the level of apoptosis. Caspase-3, Bax, Bcl-2, p AKT, AKT, Protein expression level. Results: The results of U251 and U87 cells were consistent. Compared with the control group, the proliferation and migration ability of PNS group were significantly decreased (P <0.05); the apoptosis rate was significantly increased (P <0.05); the protein expression of Bcl-2 was significantly decreased (P <0.05 ), While the expressions of Bax and Caspase-3 were significantly up-regulated (P <0.05). The protein expressions of p-Akt and p-mTOR were significantly decreased (P <0.05). Conclusion: PNS can inhibit the proliferation and migration of U251 and U87 cells and promote apoptosis, which may be related to the inhibition of PI3K-Akt-mTOR signaling pathway.