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目的分析T3溶液诱导甲亢阴虚型模型大鼠后血清中内源性代谢物的变化,初步探讨玉竹多糖预防甲亢阴虚的作用机制。方法将SD大鼠随机分为正常组,模型组,玉竹多糖组(400 mg·kg~(-1));利用注射T3溶液于大鼠腹腔(500μg·kg~(-1))连续1周建立甲亢阴虚模型,测定血浆中cAMP、cGMP、血清中T3、T4等指标。采用GC-MS技术分析大鼠血清代谢物图谱,运用软件Simca-P12.0进行多元统计分析。结果玉竹多糖可以显著降低模型动物的T3、T4、cAMP、cAMP/c GMP含量,升高cGMP含量,对甲亢阴虚型大鼠具有一定的改善作用。PCA得分图显示正常组、模型组和玉竹组的代谢轮廓有明显差别,通过偏最小二乘-判别分析(PLS-DA)对样品进行进一步分型,根据模型的变量重要性因子(VIP值)结果筛选差异代谢物,鉴定出9种内源性代谢物为生物标记物。结论研究表明,玉竹多糖干预的甲亢阴虚型大鼠整体代谢轮廓趋向正常水平,通过调节糖代谢、能量代谢、氨基酸代谢及脂类代谢等对甲亢阴虚发挥预防作用,其所揭示的代谢途径变化将为阴虚诊断及治疗提供一定的依据。
Objective To analyze the changes of serum endogenous metabolites in hyperthyroidism-yin-deficiency model rats induced by T3 solution and to explore the mechanism of the effect of Polygonatum y polysaccharide on preventing yin deficiency in hyperthyroidism. Methods SD rats were randomly divided into normal group, model group and Polygonatum polysaccharides group (400 mg · kg -1). The rats were injected intraperitoneally (500 μg · kg -1) Zhou model of hyperthyroidism Yin, determination of plasma cAMP, cGMP, serum T3, T4 and other indicators. Serum metabolites profiles were analyzed by GC-MS and multivariate statistical analysis was performed using software Simca-P12.0. Results Polygonatum polysaccharide can significantly reduce the model animal T3, T4, cAMP, cAMP / c GMP content, increased cGMP content of hyperthyroidism Yin-type rats have some improvement. PCA score showed that the metabolic profile of normal group, model group and Yuzhu group were significantly different. The samples were further typed by partial least-squares-discriminant analysis (PLS-DA). According to the model’s variable importance factor (VIP value ) Results Screening for differential metabolites, nine endogenous metabolites were identified as biomarkers. CONCLUSIONS: The results showed that the whole metabolic profile of hyperthyroidism-yin-deficiency rats tended to be normal with the intervention of Polygonatum Polysaccharides, which could prevent the hyperthyroidism by regulating glucose metabolism, energy metabolism, amino acid metabolism and lipid metabolism. The revealed metabolism Path changes will provide a basis for the diagnosis and treatment of yin deficiency.