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自由基在辐射损伤中起着重要作用。应用抗氧化剂可在一定程度上清除自由基,从而减轻辐射损伤。该研究应用荧光分光光度法测定了照后小鼠组织和血清中脂质过氧化代谢产物MDA的含量变化,以此来研究其剂量效应关系,以及抗氧化剂Vit E和DMSO对照后小鼠的保护作用,探讨抗氧化剂在临床方面的应用。结果表明:(1)3.0Gy照后12～24小时,MDA含量最高,然后逐渐下降;(2)MDA随照射剂量的增大而增高,即MDA可很好地反映机体损伤情况;(3)Vit E和DMSO都能明显地降低照后小鼠体内MDA的水平,DMSO的作用大于Vit E,两者的最佳用量分别为:10mg/g体重和0.25mg/g体重;(4)DMSO和Vit E联合应用,其防护效果优于单独使用的效果。
Free radicals play an important role in radiation damage. Antioxidants can be used to some extent to eliminate free radicals, thereby reducing radiation damage. In this study, fluorescence spectrophotometry was used to determine the changes of lipid peroxidation metabolites (MDA) in mice tissues and serum after irradiation to study the dose-response relationship and the protective effect of antioxidants Vit E and DMSO Role, to explore the clinical application of antioxidants. The results showed that: (1) MDA content was highest at 12-24 hours after 3.0Gy irradiation and then gradually decreased; (2) MDA increased with the increase of irradiation dose, that is, MDA could well reflect the damage of the organism; (3) Both Vit E and DMSO significantly reduced the level of MDA in mice after irradiation, and the effect of DMSO was greater than that of Vit E. The optimum dosages of both were 10 mg / g body weight and 0.25 mg / g body weight respectively. (4) Vit E joint application, the protective effect is superior to the effect alone.