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目的:对毛茛科乌头属植物滇北乌头的块根进行化学成分研究。方法:滇北乌头的干燥块根粉碎后用甲醇渗漉提取,回收溶剂所得浸膏用1.5%HCl溶解,乙酸乙酯萃取,酸水溶液用5% NaOH调至pH为9,乙酸乙酯萃取,得总生物碱粗提物。粗提物用各种柱色谱进行分离纯化,经光谱数据(~1H-NMR,~(13)C-NMR,MS)进行结构鉴定。结果:分离鉴定18个二萜生物碱,分别为:14-O-acetylsachaconitine(1),franchetine(2),crassicaudine(3),indaconoitine(4),14-benzoyl chasmanine(5),14-Oacetyltalatisamine(6),talatisamine(7),chasmannine(8),crassicauline A(9),bikhaconine(10),13,15-dideoxyaconitine(11),crassicautine(12),kongboensine(13),liljestrandisine(14),ludaconitine(15),8-deacetyl-yunaconitine(16),yunaconitine(17),ouvrardiantine(18)。结论:首次对滇北乌头进行化学成分研究,共分离鉴定18个二萜生物碱。
Objective: To study the chemical constituents of the roots of Aconiti in Yunnan Province. Methods: The dried roots of Aconitum yunnanensis were crushed and then extracted with methanol percolation. After the solvent was recovered, the resulting extract was dissolved in 1.5% HCl and extracted with ethyl acetate. The aqueous acid solution was adjusted to pH 9 with 5% NaOH and extracted with ethyl acetate. Total alkaloid crude extract. The crude extracts were separated and purified by various column chromatography and identified by spectroscopic data (~ 1H-NMR, ~ (13) C-NMR, MS). RESULTS: Eighteen diterpene alkaloids were isolated and identified as 14-O-acetylsachaconitine (1), franchetine (2), crassicaudine (3), indaconoitine (4), 14-benzoyl chasmanine 6) talatisamine (7) chasmannine (8) crassicauline A (9) bikhaconine (10) 13,15- dideoxyaconitine (11) crassicautine (12) kongboensine (13) liljestrandisine (14) ludaconitine 15), 8-deacetyl-yunaconitine (16), yunaconitine (17), ouvrardiantine (18). Conclusion: The chemical constituents of Aconitum nigrum were studied for the first time, and 18 diterpenoid alkaloids were isolated and identified.