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【目的】钙依赖蛋白激酶(CPKs)是一类依赖于Ca~(2+)的丝氨酸/苏氨酸蛋白激酶,仅在植物和部分低等生物中存在。Ca~(2+)作为第二信使,在植物生长发育和抗逆应答过程中起着重要作用。研究玉米Zm CPK9基因在幼苗期不同逆境胁迫下的动态表达,为分析该基因在玉米逆境应答中的功能和机制奠定基础。【方法】利用生物信息学方法,分析Zm CPK9基因序列特征,采用Real-time PCR技术分析Zm CPK9在干旱、低温、盐和ABA诱导下的表达特征。【结果】利用生物信息学方法从玉米基因组中鉴定出CPK基因,命名为ZmCPK9。序列分析表明Zm CPK9 c DNA序列长1 548 bp,编码515个氨基酸。生物信息学分析显示,在蛋白质序列和结构上该基因与其他物种CPK基因一样非常保守。Real-time PCR的结果表明,在干旱、低温、盐和ABA诱导下,Zm CPK9表达量均有所上调。【结论】Zm CPK9是玉米CPKs基因家族中一个新的成员,对干旱、低温、盐和ABA等非生物胁迫具有应答反应,推测Zm CPK9对植物防御非生物胁迫具有一定作用。
【Objective】 Calcium-dependent protein kinases (CPKs) are serine / threonine protein kinases that depend on Ca 2+ and exist only in plants and some lower organisms. Ca 2+ as second messenger plays an important role in plant growth and development and in response to stress. Studying the dynamic expression of Zm CPK9 gene under different stress conditions in seedling stage lays the foundation for analyzing the function and mechanism of this gene in response to maize stress. 【Method】 The sequence of Zm CPK9 gene was analyzed by bioinformatics methods. The expression characteristics of Zm CPK9 under drought, hypothermia, salt and ABA were analyzed by Real-time PCR. 【Result】 The CPK gene was identified from corn genome by bioinformatics method and named as ZmCPK9. Sequence analysis showed that the DNA sequence of Zm CPK9c was 1 548 bp, encoding 515 amino acids. Bioinformatics analysis revealed that the gene is as conserved as the CPK genes of other species in terms of protein sequence and structure. The results of Real-time PCR showed that the expression of Zm CPK9 was up-regulated under drought, hypothermia, salt and ABA induction. 【Conclusion】 Zm CPK9 is a new member of the CPKs gene family in maize, which responds to abiotic stresses such as drought, hypothermia, salt and ABA. It is speculated that Zm CPK9 may play a role in plant defense against abiotic stress.