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目的:检测胆囊腺癌组织中趋化因子MCP-1和MIP-1α的表达、TAM计数并探讨其临床病理意义。方法:收集中南大学湘雅二医院及湖南省人民医院近五年胆囊腺癌手术切除标本36例及慢性胆囊炎手术切除标本10例,采用原位分子杂交方法检测MCP-1和MIP-1α的表达,免疫组化法进行TAM计数。结果:胆囊腺癌组织中MCP-1、MIP-1αmRNA表达阳性率及评分均明显高于慢性胆囊炎(P<0.01);高分化胆囊腺癌中二者的阳性率及评分均低于低分化胆囊腺癌,其中MCP-1 mRNA比较有显著性差异(P<0.05);MCP-1、MIP-1αmRNA的表达呈显著正相关。胆囊腺癌组织MCP-1 mRNA表达阳性率及其评分与侵犯胆总管及发生淋巴结转移显著相关;MIP-1αmRNA表达阳性率及其评分与侵犯肝脏显著相关。胆囊腺癌组织中,TAM计数(24.89±0.84)明显高于慢性胆囊炎组织(16.19±0.66),有显著性差异(P<0.01)。TAM与MCP-1、MIP-1αmRNA表达评分值均呈显著正相关(r分别为0.580,0.567)。MCP-1mRNA与MIP-1αmRNA评分值之间呈显著正相关(r=0.638)。结论:MCP-1、MIP-1α的表达增加及TAM计数升高可能调控和影响胆囊癌的发生和发展,MCP-1、MIP-1α可能促进TAM向胆囊癌组织迁移浸润。
OBJECTIVE: To detect the expressions of chemokines MCP-1 and MIP-1α in gallbladder adenocarcinoma and to investigate the clinicopathological significance of TAM. Methods: Thirty-six cases of gallbladder adenocarcinoma and 10 cases of chronic cholecystitis were collected from Xiangya Second Hospital of Central South University and Hunan Provincial People’s Hospital in recent five years. In situ hybridization was used to detect the expression of MCP-1 and MIP-1α Expression, immunohistochemistry TAM count. Results: The positive rates and scores of MCP-1 and MIP-1αmRNA in gallbladder adenocarcinoma were significantly higher than those in chronic cholecystitis (P <0.01). The positive rates and scores of both MCP-1 and MIP-1α mRNA in gallbladder adenocarcinoma were lower than those in poorly differentiated gallbladder adenocarcinoma The expression of MCP-1 and MIP-1αmRNA was significantly positive correlated with the expression of MCP-1 mRNA (P <0.05). The positive rate of MCP-1 mRNA in gallbladder adenocarcinoma and its score were significantly correlated with infiltration of the common bile duct and lymph node metastasis. The positive rate of MIP-1αmRNA expression and its score were significantly correlated with liver invasion. In gallbladder adenocarcinoma, the TAM count (24.89 ± 0.84) was significantly higher than that of chronic cholecystitis (16.19 ± 0.66), with significant difference (P <0.01). There was a significant positive correlation between TAM and MCP-1 and MIP-1α mRNA expression (r = 0.580 and 0.567, respectively). There was a significant positive correlation between MCP-1 mRNA and MIP-1α mRNA score (r = 0.638). CONCLUSION: The increased expression of MCP-1 and MIP-1α and the increase of TAM counts may regulate and influence the occurrence and development of gallbladder carcinomas. MCP-1 and MIP-1α may promote the migration and invasion of TAM into gallbladder carcinomas.