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目的 促进培养内皮细胞 (EC)分化 ,增强 EC对流体剪切力的抵抗。 方法 利用改进的流室装置 ,通过精密蠕动泵提供剪切力 ,同时提供静态培养所需的其它条件 ,建立体外的 EC流动培养模型 ,按流动培养增加剪切力的方式分为 A组、B组和 C组 ,并筛选出最佳的流动培养模式。 结果 EC在流动培养下生长良好 ,血管 EC被拉长 ,其长轴方向与流场方向趋于一致。不同流动培养方式作用下 ,EC能适应剪切力递增式 (A组 )和阶跃式 (B组 )增加的作用 ,直接暴露于较大的剪切力作用下发生较明显的细胞脱落 (C组 )。 结论 我们建立的模型能相对地接近模拟体内环境 ,阶跃式增加剪切力在流动培养中方便、切实可行。
Objective To promote endothelial cell (EC) differentiation and enhance EC resistance to fluid shear. Methods The improved flow chamber device was used to provide the shear force by the precision peristaltic pump. At the same time, other conditions needed for static culture were provided. The EC flow culture model was established in vitro and divided into group A, B Group and group C, and screened the best flow culture mode. Results The EC grew well under flowing culture, the EC of the blood vessel was elongated, and the direction of the long axis and the flow field tended to be the same. EC could adapt to increasing shear force (group A) and step-type (group B) under the action of different flow culture modes, and more obvious cell shedding occurred under direct exposure to larger shear force (C group). Conclusion Our model can be relatively close to the simulation of the body environment, step-by-step increase in shear flow in the flow of convenient, practical.