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目的 :研究通过损毁脑干中缝背核 (DR) ,探讨中缝背核一氧化氮合酶 (NOS)阳性神经元是否投射分布于大脑皮质NOS阳性神经元。方法 :将 16只SD雄性成年大鼠分为实验组与对照组。对实验组动物DR微量注射喹啉酸 ,饲养 1周 ,灌注固定 ,然后将大脑及脑干作冠状冷冻切片 ,NADPH d组化染色。结果 :实验组动物的中缝背核被有效损毁 ,其NOS阳性神经元的数量减少了5 9 .1% (P <0 .0 0 1)。大脑皮质NOS阳性纤维终末减少了 2 8.2 % (P <0 .0 5 ) ,与大脑皮质NOS神经元构成接触的NOS阳性纤维终末减少了 3 3 .9% (P <0 .0 5 )。结论 :位于中缝背核的NOS阳性神经元投射分布于大脑皮质NOS神经元。推测中缝背核的NOS阳性神经元可能通过大脑皮质NOS阳性神经元间接对皮质脑血流量起调节作用。
AIM: To investigate whether NOS positive neurons in the dorsal raphe nucleus distribute in cerebral cortex by projecting the dorsal raphe nucleus (DR). Methods: 16 SD male adult rats were divided into experimental group and control group. The animals in the experimental group were injected microinjected with quinoline acid for 1 week and perfused for fixation. Then the brainstem and brainstem were subjected to coronary cryosection and then to NADPH d staining. Results: The dorsal raphe nucleus of experimental group was effectively destroyed, and the number of NOS-positive neurons decreased by 59.1% (P <0.01). The number of NOS positive fibers in the cerebral cortex decreased by 8.22% (P <0.05), and the number of NOS positive fibers that contacted the cerebral cortex NOS neurons decreased by 33.9% (P <0.05) . CONCLUSIONS: NOS-positive neurons located in the dorsal raphe nucleus project in the NOS neurons of the cerebral cortex. It is speculated that the NOS positive neurons in the dorsal raphe nuclei may play an indirect role in regulating cortical cerebral blood flow through NOS positive neurons in the cerebral cortex.