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目的 :研究肠道非霍奇金恶性淋巴瘤的 EB病毒感染、p5 3、p2 1ras蛋白表达及其相关性。方法 :应用单克隆抗体 CD4 5RO、CD2 0 、CD79a、CD4 5RA、CD56 、L CA、CD30 、CD1 5、CD5、CD8、TIA- 1、Gram B、p5 3、p2 1;多克隆抗体 CD3、κ、λ对瘤细胞进行免疫组化标记 ,用 SABC免疫组化的方法检测瘤细胞 p5 3、p2 1ras基因的表达及 EB病毒寡核苷酸探针 (EBER)原位杂交 ,观察了 4 0例 NHL 的免疫表型、EBV感染情况及 p5 3、p2 1ras蛋白的表达。结果 :4 0例中 ,经免疫组化证实 6例为 T细胞淋巴瘤(15 % ) ,34例为 B细胞淋巴瘤 (85 % )。T细胞淋巴瘤为外周 T细胞性和肠病型 T细胞性淋巴瘤。B细胞淋巴瘤 9例为弥漫性大细胞性淋巴瘤 2 3.5 % ,2 5例为边缘区 B细胞性淋巴瘤 (76 .5 % )。EBV-ERBR原位杂交 6 / 4 0例有阳性表达 ,均为 T细胞淋巴瘤 ,阳性细胞占肿瘤细胞的 30 %～ 80 %。 p5 3的表达共有 2 5例 ,2 1例有 p2 1ras的表达。有 16例同时检出 p5 3和 p2 1ras的表达。结论 :肠道淋巴瘤以边缘区 B细胞淋巴瘤多发临床为惰性。如为 T细胞性淋巴瘤 ,更多见的是侵袭性 ,且 T细胞淋巴瘤与 EBV相关性较高 B细胞淋巴瘤则无相关性。p5 3的表达与 EBV感染无明显相关性 ,而 p2 1ras的表达与 EBV感染似有关系。
Objective : To study the EB virus infection, p53 and p21ras protein expression and their correlation in intestinal non-Hodgkin’s lymphoma. METHODS: Monoclonal antibodies CD4 5RO, CD20, CD79a, CD4 5RA, CD56, LCA, CD30, CD15, CD5, CD8, TIA-1, Gram B, p53, p21; polyclonal antibodies CD3, κ The tumor cells were immunohistochemically labeled with λ, and the expression of p53 and p21ras genes in tumor cells was detected by SABC immunohistochemistry and in situ hybridization with EBER EBV oligonucleotide probe (EBER). A total of 40 cases were observed. NHL immunophenotype, EBV infection, and expression of p53 and p21ras proteins. RESULTS: In 40 cases, 6 cases were confirmed as T-cell lymphoma (15 %) and 34 cases were B-cell lymphoma (85 %) by immunohistochemistry. T-cell lymphomas are peripheral T-cell and intestinal-type T-cell lymphomas. 9 of the B-cell lymphomas were diffuse large cell lymphomas, 2 3.5%, and 25 were marginal B-cell lymphomas (76.5%). EBV-ERBR in situ hybridization 6/40 positive expression, are T-cell lymphoma, positive cells accounted for 30% to 80% of tumor cells. There were 25 cases of p53 expression and 21 cases had p21ras expression. In 16 cases, the expression of p53 and p21ras was also detected. Conclusion: Intestinal lymphomas are clinically inert with marginal B-cell lymphoma. If it is T-cell lymphoma, it is more likely to be invasive, and there is no correlation between T-cell lymphoma and EBV-related B-cell lymphoma. There was no significant correlation between the expression of p53 and EBV infection, but the expression of p21ras was similar to that of EBV infection.