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目的观察泥敷、堆敷灭螺中氯硝柳胺在土壤中的含量和分布、随时间的变化情况及其与灭螺效果的关系。方法土壤样品分别采自四川省普格县的4个地区,经超声萃取、离心、浓缩后用高效液相色谱测定其含量;灭螺试验分别计算3d和7d钉螺死亡率。结果各组土壤中的氯硝柳胺含量分布很不均匀,采用堆敷灭螺的特兹乡甲木村先锋组、长寿村新建组表层土与深层土氯硝柳胺含量差异无统计学意义(P>0.05);采用泥敷灭螺的荞窝镇安木足村五组与特兹乡长寿村米石组因间隔时间不同,土壤中氯硝柳胺含量差异有统计学意义(P=0);堆敷灭螺法在灭螺13个月后,土壤中仍残留一些氯硝柳胺,并且还有一定的灭螺效果,钉螺死亡率最高达21.26%;钉螺3、7d死亡率与土壤中氯硝柳胺含量的Spearman相关系数分别为0.354、0.306(P均<0.05)。结论堆敷灭螺法因结合了环境改造,灭螺效果优于泥敷法;2种灭螺方法的氯硝柳胺在土壤中的分布不均匀,其含量随着时间的推移逐渐降低,灭螺效果与氯硝柳胺含量有一定的正相关关系。
Objective To observe the content and distribution of niclosamide in soil and its change with time and its relationship with snail-killing effect in mud pack and pile-packing. Methods Soil samples were collected from 4 districts in Puge County, Sichuan Province. The samples were extracted by ultrasound, centrifuged and concentrated. The contents of soil samples were determined by high performance liquid chromatography. The snail mortality was calculated on 3d and 7d respectively. Results The content of niclosamide in soil of each group was very uneven. There was no significant difference in the content of niclosamide between the surface soil and the deep soil in the newly constructed group of Changshou Village, P> 0.05). There were significant differences in the concentrations of niclosamide in the soil between the five groups of Anmuzuicu and the Mishishan of Changshou village, ); Heaping off snail method in the snail for 13 months, the soil is still some niclosamide residues, and there is a certain effect of snail snail, snail mortality up to 21.26%; snail 3,7d mortality and soil The Spearman correlation coefficients of niclosamide content were 0.354 and 0.306, respectively (all P <0.05). Conclusion The results showed that the two methods of molluscicidal treatment of niclosamide were unevenly distributed in the soil, and their content decreased gradually with the passage of time. Spiro effect and niclosamide content have a certain positive correlation.