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背景:课题计划从神经细胞替代、促进内源性神经干细胞增殖和分化、保护神经元、促进突触重建以及减轻脑白质损伤等方面来探讨脐血间充质干细胞系统移植对新生大鼠缺氧缺血脑损伤后神经功能的修复作用及其机制。目的:观察脐血间充质干细胞由静脉途径移植透过血脑屏障进入脑组织内,对新生大鼠缺氧缺血性脑损伤后脑功能修复的影响。方法:7d龄SD新生鼠分为3组:假手术组仅分离出左侧颈总动脉而不结扎;缺氧缺血脑损伤组制备缺氧缺血脑损伤模型;细胞移植组在缺氧缺血性脑损伤后第8天尾静脉移植人脐血间充质干细胞,前两组尾静脉注射等量的生理盐水。结果与结论:免疫荧光观察显示移植后5周脐血间充质干细胞迁移到海马,Nissl染色结果显示脐血间充质干细胞移植后,左侧海马DG区锥体细胞尼氏小体明显增加,提示间充质干细胞移植后可分化为神经元。行为学测试结果显示:与假手术组相比,缺氧缺血脑损伤组在T迷宫实验中,自发改变率下降,在放射形迷宫中觅水时间延长,错误次数及重复次数明显增加(P<0.05);脐血间充质干细胞静脉移植5周后,上述行为学指标均显著改善(P<0.05)。提示脐血间充质干细胞静脉移植治疗明显改善和提高了缺氧缺血脑损伤大鼠远期的学习记忆和空间辨别能力。
Background: The project plans to explore the effects of umbilical cord blood mesenchymal stem cell transplantation on neonatal rat hypoxia by replacing neurons, promoting the proliferation and differentiation of endogenous neural stem cells, protecting neurons, promoting synaptic reconstruction and reducing white matter damage. Neurofunctional repair and its mechanism after ischemic brain injury. OBJECTIVE: To observe the effects of umbilical cord blood mesenchymal stem cells transplanted through the blood-brain barrier into the brain tissue by intravenous route on brain function recovery after hypoxic-ischemic brain damage in neonatal rats. Methods: The 7-day-old SD neonatal rats were divided into 3 groups: the sham-operated group was isolated only from the left common carotid artery without ligation; the hypoxic-ischemic brain damage model was established by hypoxic-ischemic brain damage group; On the 8th day after the traumatic brain injury, human umbilical cord blood mesenchymal stem cells were transplanted into caudal vein, and the same two groups were injected with the same amount of saline. RESULTS AND CONCLUSION: Immunofluorescence showed that UCB-MSCs migrated to the hippocampus 5 weeks after transplantation. The results of Nissl staining showed that after transplantation of UCB-MSCs, the number of Nissl bodies of DG in pyramidal cells of left hippocampus significantly increased, Prompted mesenchymal stem cells can differentiate into neurons after transplantation. The result of behavioral test showed that compared with the sham operation group, spontaneous change rate of hypoxic-ischemic brain injury group decreased in T-maze test, the time of finding water in radial maze was prolonged, the error times and repetition times increased obviously (P <0.05). After umbilical cord blood mesenchymal stem cells were transplanted for 5 weeks, the behavioral indexes were significantly improved (P <0.05). Prompt umbilical cord vein mesenchymal stem cells vein graft therapy significantly improved and improved hypoxic-ischemic brain damage in rats long-term learning and memory and spatial discrimination.