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采用 m RNA差异显示技术对受 GA3诱导的水稻伸长相关基因进行了分离和鉴定。经 10 0个引物组合差异显示 ,在获得 2 1个受 GA3诱导表达的 c DNA片段的基础上 ,对其中的 GA15 b片段克隆进行了序列测定。其序列经国际联网BLAST查询表明 GA15 b为伸展蛋白类似蛋白的候选基因片段。定量 PCR进一步确证 GA15 b受 GA3所诱导。Southern杂交表明 GA15 b以多拷贝形式存在
The rice elongation-related genes induced by GA3 were isolated and identified by m RNA differential display. The difference of 10 primer combinations showed that the cloned GA15 b fragment was sequenced after obtaining 21 c DNA fragments induced by GA3. The sequence was searched by an internet-linked BLAST to show that GA15b is a candidate gene fragment for extensin-like proteins. Quantitative PCR further confirmed that GA15b is induced by GA3. Southern blotting indicates that GA15b is present in multiple copies