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目的研究胃癌干细胞样细胞的初步分选与鉴定。方法选取20例胃癌患者和15例正常者组织细胞采用流式细胞分离纯化、22CKK-8细胞生长曲线测定、流式细胞仪检测的方式对其进行临床检测和分析。结果CD_(44)~+、CD_(24)~+细胞在胃癌组织中的比率组间比较差异有统计学意义(P<0.05),在胃癌与正常胃组织中抗原荧光表达量组间比较差异有统计学意义(P<0.05);细胞体外生长曲线差异有统计学意义(P<0.05);CD_(44)~+、CD_(24)~+细胞凋亡率显著低于Total组(P<0.01),其增殖指数与Total组比较差异无统计学意义(P>0.05),S和G2/M期较Total细胞轻度增加,G0/G1轻度减少。结论 CD_(44)~+、CD_(24)~+细胞群可能在胃肿瘤的发生、发展中起重要作用,其可能富含胃癌干细胞样细胞;CD_(44)、CD_(24)可能是胃癌细胞的干细胞标志物。
Objective To study the preliminary sorting and identification of gastric cancer stem cell-like cells. Methods Tissue cells of 20 patients with gastric cancer and 15 normal controls were selected and purified by flow cytometry. The growth curve of 22CKK-8 cells was determined by flow cytometry. The clinical samples were analyzed by flow cytometry. Results There was significant difference between the rates of CD44 + cells and CD24 + cells in gastric cancer tissues (P <0.05), and there was significant difference in the expression of antigen between gastric cancer and normal gastric tissues (P <0.05). The growth curve of cells in vitro showed a significant difference (P <0.05). The apoptotic rates of CD44 +, CD24 + cells were significantly lower than those in Total group (P < 0.01). The proliferation index was not significantly different from Total group (P> 0.05). S and G2 / M increased slightly compared with Total and G0 / G1 decreased slightly. Conclusions CD44 +, CD24 + cell population may play an important role in the development and progression of gastric neoplasms, which may be enriched in gastric cancer stem cell - like cells. CD44 (44) and CD24 (24) Stem cell markers of cells.