论文部分内容阅读
应用制备的单克隆抗体SC_2A及ABC免疫组化法检测了338例胃粘膜异型增生标本SC_2A-Ag表达情况,并进行形态分级.对其中的224例进行6～74个月(平均25个月)的随访.结果表明,癌变组异型增生31例中SC_2A-Ag呈阳性表达者19例(61.3%),未癌变组35例中阳性表达者11例(31.4%),两组阳性率差异有非常显著意义(P<0.01),其判断异型增生癌变的敏感性为61.3%,特异性为68.6%,阳性预值为63.3%,阴性预值为66.7%.若以SC_2A-Ag和Ⅱ～Ⅲ度异型增生两项指标平行联合判断,其敏感性可提高至71.0%;若以两项指标系列联合判断,其特异性可提高至82.9%,较形态分级单独判断为优(P<0.05).应用SC_2A抗原与形态分级联合判断胃粘膜异型增生癌变,有利于提高早期胃癌的检出率.
The expression of SC_2A-Ag in 338 gastric mucosal dysplasia specimens was detected by immunohistochemical method using prepared monoclonal antibodies SC_2A and ABC, and morphological grading was performed. 224 of them were performed for 6 to 74 months (average 25 months). The follow-up results showed that 19 cases (61.3%) of SC_2A-Ag were positively expressed in 31 cases of dysplasia in the cancerous group, and 11 cases (31.4%) were positive in the noncancerous group. There was a very significant difference between the two groups in the positive rate. Significant (P<0.01), its sensitivity to the diagnosis of dysplasia was 61.3%, specificity was 68.6%, positive predictive value was 63.3%, negative predictive value was 66.7%. If SC_2A-Ag and II～III degree When the two indicators of dysplasia were judged in parallel and jointly, the sensitivity could be increased to 71.0%; if judged by the combination of the two indicators, the specificity could be improved to 82.9%, which was better than the morphology classification alone (P<0.05). The SC_2A antigen combined with morphology and grade to judge gastric mucosa dysplasia and cancerous, is helpful to improve the detection rate of early gastric cancer.