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目的:用大孔吸附树脂层析技术分离丹参酮ⅡA。方法:通过HPD-100树脂对丹参酮ⅡA的吸附穿透曲线与静态实验所得饱和吸附量来确定上样量,进行乙醇梯度洗脱,用高效液相色谱检测丹参酮ⅡA,初步确定树脂的洗脱条件进而分析出分离的优化方案。结果:丹参酮ⅡA的分离纯化提取工艺为:丹参酮提取液,用HPD-100大孔吸附树脂,80%乙醇洗脱。结论:该工艺可以将丹参酮ⅡA的浓度由提取液中的17%左右富集到42%左右,浓度提高了2.5倍左右。
Objective: Separation of tanshinone IIA by macroporous adsorption resin chromatography. Methods: The loading amount of tanshinone IIA adsorbed by HPD-100 resin was determined with the saturated adsorption amount obtained by static experiments. Ethanol gradient elution was performed, and tanshinone IIA was detected by HPLC to determine the elution conditions of the resin. Then analyze the optimized optimization scheme. RESULTS: The separation and purification process of tanshinone IIA was as follows: Tanshinone extract was eluted with HPD-100 macroporous adsorption resin and 80% ethanol. Conclusion: The process can enrich the concentration of tanshinone IIA from about 17% in the extract to about 42%, and the concentration is increased by about 2.5 times.