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应用茎尖嫁接及热处理与茎尖嫁接相结合的技术,对我国柑桔的主要病毒类病害(黄龙病、衰退病、裂皮病、碎叶病)进行脱除病原研究。结果表明:黄龙病、衰退病、裂皮病平均脱除率分别为100% 、80.6% 和54.1% 。单用茎尖嫁接对碎叶病脱除无效,但用热处理与茎尖嫁接相结合的方法进行脱除,可以收到理想效果。同时,研究了嫁接前切口处加激素、茎尖大小、适宜砧木苗龄、砧木带与不带子叶、再嫁接等对提高茎尖嫁接成活率的影响。
Applying techniques of shoot tip grafting and heat treatment combined with stem-tip grafting, the pathogenicity of the major virus diseases of citrus (Huanglongbing, Recession, Split-skin disease and Leaf-broken disease) in China was studied. The results showed that the average rates of yellow dragon’s disease, recession and split skin disease were 100%, 80.6% and 54.1% respectively. Single root tip grafting on the removal of broken leaf disease is invalid, but with heat treatment and shoot tip grafting method for removal, you can receive the desired effect. At the same time, the effects of adding hormones, shoot tip size, appropriate rootstock seedling age, rootstocks with and without cotyledons, and then grafting on improving the survival rate of shoots after grafting were studied.