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目的:研究白藜芦醇(resveratrol,Res)对酒精性肝损伤后炎性因子水平的影响和NF-κB信号通路的变化。方法:大鼠灌胃给予55度红星二锅头复制肝损伤模型。随机分为正常对照组、肝损伤模型对照组、Res低剂量组(25mg/kg)、Res中剂量组(50mg/kg)、Res高剂量组(100mg/kg)、联苯双酯组成(100mg/kg),每日给药2次,连续7d。以Elisa试剂盒分别测定血清TNF-α、IL-6、ICAM-1含量和肝组织NF-κBp65、磷酸化IκB-α水平。结果:与正常对照组相比,酒精性肝损伤模型大鼠血清中TNF-α、IL-6、ICAM-1水平和肝组织NF-κB、磷酸化IκB-α水平显著升高。与模型对照组相比,Res显著降低血清TNF-α、IL-6、ICAM-1含量,抑制肝组织NF-κB、磷酸化IκB-α水平。结论:Res通过抑制NF-κB、降低炎性因子水平,对于酒精性肝损伤的肝功能具有保护作用。
Objective: To investigate the effect of resveratrol (Res) on inflammatory cytokines levels and the changes of NF-κB signaling pathway in alcoholic liver injury. Methods: Rats were intragastrically given 55 degree red star Erguotou liver injury model. Were randomly divided into normal control group, liver injury model control group, Res low dose group (25mg / kg), Res medium dose group (50mg / kg), Res high dose group (100mg / kg), bifendate composition / kg), administered twice daily for 7d. Serum levels of TNF-α, IL-6 and ICAM-1, NF-κBp65 and phosphorylated IκB-α were measured by Elisa kit. Results: Compared with the normal control group, serum levels of TNF-α, IL-6 and ICAM-1 and the levels of NF-κB and phosphorylated IκB-α in alcoholic liver injury model rats were significantly increased. Compared with the model control group, Res significantly decreased the levels of serum TNF-α, IL-6 and ICAM-1, and inhibited the levels of NF-κB and phosphorylated IκB-α in liver tissues. Conclusion: Res can protect liver function of alcoholic liver injury by inhibiting NF-κB and decreasing the level of inflammatory cytokines.