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HePler氏所著的临床检验方法手册一书内会提到伊红酒精溶液加盐酸后能增加Harris氏苏木素在染组织切片时的染色性能,所用伊红盐酸酒精的配制如下: 伊红 0.25克 0.1当量的盐酸 4毫升 90%酒精 100毫升作者依法配制此伊红盐酸酒精溶液时将0.1当量的盐酸误为一个当量,发现用误配之溶液处理涂片后能使品篮迅速染色法染幼丝虫的性能增进,其染色结果且较用0.1当量的盐酸所配制的伊红酸酒精溶液为优,此一偶然的观察,促
HePler’s book Clinical Tests Handbook mentions that eosin alcohol solution plus hydrochloric acid can increase the dyeing performance of Harris’s hematoxylin when it is stained. The eosin hydrochloride is formulated as follows: Eosin 0.25 g 0.1 Equivalent hydrochloric acid 4 ml 90% ethanol 100 ml The author formulates this eosin hydrochloride alcohol solution when 0.1 equivalent of hydrochloric acid mistaken for an equivalent and found that the misfit with the solution after the smear can make the basket quickly dyed silk Insect performance improvement, the staining results and more than 0.1 eq of hydrochloric acid formulated eosin alcohol solution is excellent, the chance to observe and promote