The argyrophilic staining technique was used in differential diagnosis between non-odgkin’s lymphoraa (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases) and high malignant (11 cases) groups. The mumber and the area of Ag nucleolar organizer region (AgNORs) in 100 cells of each case were examined by both IBAS-200 cytoimage analyser and microscope. The results showed that there was significant difference in the number, but not in area, of AgNOR granules between RH and low malignant group of NHL (P< 0.001). In NHL, the significant differences in the number and area of AgNORs were found between each groups (P<0.05 or P< 0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of AgNOR combining wit The argyrophilic staining technique was used in differential diagnosis between non-odgkin’s lymphoma (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases The mumber and the area of ​​Ag nucleolar organizer region (AgNORs) in 100 cells of each case were both examined by both IBAS-200 cytoimage analyzer and microscope. The results showed that there was significant difference in The numbers, but not in area, of AgNOR granules between RH and low malignant group of NHL (P <0.001). In NHL, the significant differences in the number and area of ​​AgNORs were found between each groups (P <0.05 or P < 0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of ​​the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of A gNOR combining wit