胰岛素是调节细胞代谢、促进细胞生长的重要因子，也是体外细胞无血清培养的必不可少的补充因子．本文测定了胰岛素不同浓度时的荧光发射强度。胰岛素荧光谱能测定胰岛素构象的变化，并能反映胰岛素生物功能的变化。 Insulin is an important factor that regulates cell metabolism and promote cell growth, and is also an indispensable supplement factor for serum-free culture of cells in vitro. This article measured the fluorescence intensity of insulin at different concentrations. Insulin fluorescence spectroscopy can measure insulin conformational changes, and can reflect changes in insulin biological function.