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探讨DNA倍体分析在胰腺占位性病变中的诊断价值.[方法]收集本院胰腺占位性病变148例,超声内镜引导下细针穿刺获取病变组织,DNA倍体自动检测分析仪对染色切片中的每个细胞核进行DNA倍体分析,并与细胞学和组织学结果对照分析.[结果]148例病变经穿刺均得到理想的标本并获得细胞学诊断,64例胰腺非肿瘤性病变无DNA倍体异常细胞,84例胰腺肿瘤性病变中发现79例DNA倍体异常细胞,阳性率94.0%(79/84),明显高于非肿瘤性病变的0.0% (0/64),胰腺肿瘤性病变的DNA倍体阳性率为94%(79/84),显著高于细胞学诊断的76.2%(64/84),其差异有统计学意义(P<0.05).[结论]DNA倍体分析可提高胰腺占位性病变检测的阳性率,对胰腺占位性病变患者的诊断提供参考价值.“,”ToexplorethediagnosticvaluesofDNAploidyanalysisinpancreaticspace-occup-yinglesions.[Methods]Atotalof148patientswithpancreaticspace-occupyinglesionsfromtheThirdXian-gyaHospital,CentralSouthUniversitywerecollected.EUS-FNA (ultrasoundendoscopicallyguidedfinalnee-dleaspiration)wereperformedinallcases.DNAploidyanalysissystem wasusedtoanalyzeDNAcontentin nucleus.[Results]EUS-FNA wassuccessfullyperformedandadequatespecimenforcytologicaldiagnosiswere obtainedin148patients.Resultsshowedthat64patients withnon-neoplasticlesionofthepancreashadno DNAploidyabnormalcellsandoutofthe84patientswithpancreaticneoplasticlesions,79caseswerefound withDNAploidyabnormalcells.Theaneuploidypositiveratewas(94.0%(79/84))in84pancreaticneoplastic lesion,whichwassignificantlyhigherthannon-neoplasticlesionofpancreas0.0% (0/64).Thedifferencewas statisticallysignificantbetweenthetwogroups(P<0.05).[Conclusion]DNAploidyanalysiscanimprovethe positivedetectionrateofpancreaticspace-occupyinglesions.Ithasclinicalguidanceforthediagnosisofpa-tientswithpancreaticspace-occupyinglesions.