利用高速逆流色谱对两株昆虫病原真菌蜡蚧霉发酵液的乙酸乙酯粗提物进行分离纯化,GC/MS对分离物进行检测.结果表明:利用高速逆流色谱经两相溶剂系统:正己烷-乙酸乙酯-甲醇-水(4∶5∶4∶5)均可从两菌株代谢产物中分离纯化得到邻苯二甲酸二丁酯,研究发现蜡蚧霉代谢产物中存在天然的邻苯二甲酸二丁酯. The crude extract of the two pathogenic fungi, waxy mildew, was separated and purified by high performance countercurrent chromatography (GC / MS). The results showed that the crude extracts were separated by high performance countercurrent chromatography - ethyl acetate - methanol - water (4: 5: 4: 5) can be isolated from the two strains of metabolites obtained dibutyl phthalate, the study found that the metabolites of waxy mildew there is a natural phthalate Dibutyl formate.