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作者研究出了黑麦草和多花黑麦草×F.donax杂种子房染色体有丝分裂计数的一种简易技术。概括说来,这个技术包括以下程序: (1)首先将子房在1℃蒸馏水中预处理16小时或在α—溴萘中预处理1 1/2小时;(2)再用3:1酒精:醋酸液中固定1—2小时;(3)在60℃ 1 N的HCl中水解10分
The authors developed a simple technique for counting mitochondrial chromosome counts in ryegrass and ryegrass × F.donax hybrids. In summary, this technique includes the following procedures: (1) Preconditioning the ovary for 16 hours in 1 ° C distilled water or 1½ hours in α-bromonaphthalene; (2) Reusing the 3: 1 alcohol : Acetic acid solution for 1-2 hours; (3) Hydrolysis in 1 N HCl at 60 ° C for 10 minutes