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目的通过检测血管内皮生长因子-C(VEGF-C)及其受体Flt-4在肝癌组织中的表达,分析它们与肝癌脉管形成及临床病理特征之间的关系,以了解VEGF-C在肝癌进程中的作用。方法采用免疫组化染色法检测62例肝细胞肝癌(HCC)组织及15例正常肝组织中VEGF-C和Flt-4的表达,并计算以CD34为标记的微血管密度(MVD)和以Flt-4为标记的淋巴管密度(LVD),分析它们相互之间及各自与HCC临床病理特征之间的关系。结果 HCC组织中VEGF-C及Flt-4的阳性表达率均明显高于正常肝组织(P<0.05,P<0.01)。HCC组织中VEGF-C的表达与有无门静脉癌栓、肿瘤组织学分化程度、术后复发及转移有关(P<0.05,P<0.01);Flt-4的表达与肿瘤组织学分化程度及术后复发有关(P<0.05,P<0.01);MVD与肿瘤大小、TNM临床分期、肿瘤组织学分化程度、有无门静脉癌栓、术后复发和转移有关(P<0.05,P<0.01);LVD与肿瘤组织学分化程度、术后复发和转移有关(P<0.05,P<0.01)。HCC组织中VEGF-C和Flt-4的表达及MVD和LVD这四者之间均两两呈正相关(P<0.01)。结论 VEGF-C和Flt-4在肝癌组织中高表达并与术后复发有关,且与MVD及LVD呈正相关,提示VEGF-C/Flt-4通路可能通过促进肝癌血管和淋巴管的生成从而影响肝癌的进展及预后。
Objective To detect the expression of vascular endothelial growth factor-C (VEGF-C) and its receptor Flt-4 in hepatocellular carcinoma (HCC), and to investigate the relationship between the expression of VEGF-C and the clinicopathological features of hepatocellular carcinoma The role of liver cancer in the process. Methods The expressions of VEGF-C and Flt-4 in 62 hepatocellular carcinoma (HCC) tissues and 15 normal liver tissues were detected by immunohistochemical staining, and the microvessel density (MVD) marked by CD34 and the expression of Flt- 4 labeled lymphatic vessel density (LVD), analysis of their relationship to each other and the clinicopathological features of HCC. Results The positive rates of VEGF-C and Flt-4 in HCC tissues were significantly higher than those in normal liver tissues (P <0.05, P <0.01). The expression of VEGF-C in HCC tissues was correlated with the presence of portal vein tumor thrombus, the degree of tumor histological differentiation, postoperative recurrence and metastasis (P <0.05, P <0.01), the expression of Flt-4 and histological differentiation degree (P <0.05, P <0.01). MVD was correlated with tumor size, TNM clinical stage, tumor histological grade, presence or absence of portal vein tumor thrombus and postoperative recurrence and metastasis (P <0.05, P <0.01). LVD was correlated with tumor histological differentiation, postoperative recurrence and metastasis (P <0.05, P <0.01). The expression of VEGF-C and Flt-4 in HCC tissues was positively correlated with MVD and LVD (P <0.01). Conclusions VEGF-C and Flt-4 are highly expressed in hepatocellular carcinoma and correlate with postoperative recurrence, and are positively correlated with MVD and LVD, suggesting that VEGF-C / Flt-4 pathway may affect hepatocellular carcinoma by promoting angiogenesis and lymphangiogenesis Progress and prognosis.