要使液相层析达到高速和高效,关键在于改进提高层析柱填充剂的结构和性能。经典技术就是使用相当大直径的柱(10～20毫米)和全多孔型的大颗粒填充剂(>100微米),此时,由于液体依赖重力或其它低压力的推动而流动,因此层析速度缓慢。使用高压泵可使分离速度增加,但还必须采用为液相层析柱特殊设计的填充剂,才能使层析分离速 For liquid chromatography to achieve high speed and high efficiency, the key is to improve the structure and performance of the column packing. The classical technique is to use relatively large diameter columns (10 to 20 mm) and full-porous large particles (> 100 μm) where the liquid flows due to gravity or other low pressure and thus the chromatographic velocity slow. The use of high-pressure pump can increase the separation speed, but also must be used for liquid chromatography column specially designed filler, chromatography separation speed