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目的: 分析胃癌中11种CT抗原(cancer/testisantigen)基因的表达分布及NY ESO 1蛋白引发的自身体液免疫应答,为胃癌的特异性肿瘤疫苗治疗提供依据。方法: 利用RT-PCR方法,检测101例胃癌患者肿瘤标本和对应正常胃黏膜中11种CT抗原基因的表达。ELISA方法检测抗NY ESO 1抗体。利用NY ESO 1的单抗E978,应用免疫组化方法检测组织切片中NY ESO 1抗原物质。结果: 101例胃癌患者中, 74. 3%的患者至少表达1种检测的CT抗原基因。12例患者表达NY ESO 1mRNA, 5例可以检测到NY ESO 1抗原蛋白以及针对NY ESO 1抗原的体液免疫反应。结论: 胃癌中表达多种CT抗原基因,表达率较高的MAGE 3,SSX 4和NY ESO 1 /LAGE 1蛋白等可作为肿瘤疫苗的备选抗原,其中NY ESO 1蛋白的免疫原性得到验证。CT抗原的多价肿瘤疫苗在胃癌治疗中具有一定的可行性。
OBJECTIVE: To analyze the expression and distribution of 11 kinds of CT antigens (cancer / testisantigen) and their own humoral immune responses induced by NY ESO 1 protein in gastric cancer and provide the basis for the treatment of gastric cancer-specific tumor vaccines. Methods: RT-PCR was used to detect the expression of 11 CT antigen genes in 101 gastric cancer samples and corresponding normal gastric mucosa. Anti-NY ESO 1 antibody was detected by ELISA. NY ESO 1 monoclonal antibody E978 was used to detect NY ESO 1 antigen in tissue sections by immunohistochemistry. Results: Among 101 gastric cancer patients, 74.3% of patients expressed at least one kind of CT antigen gene. NY ESO 1 mRNA was expressed in 12 patients, NY ESO 1 antigen protein was detectable in 5 patients, and humoral immune response against NY ESO 1 antigen was detected in 5 patients. CONCLUSIONS: MAGE 3, SSX 4 and NY ESO 1 / LAGE 1, which express a variety of CT antigen genes in gastric cancer, may be the candidate antigens for tumor vaccine. The immunogenicity of NY ESO 1 protein is verified . Multivalent tumor vaccine CT antigen in gastric cancer treatment has a certain feasibility.