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目的：研究原发性肝癌中转移相关基因ＣＤ４４ｖ与抑癌基因ＤＣＣ表达间的关系以及观察它们在原发性肝癌侵袭转移中的作用。方法：应用ＲＴ－ＰＣＲ检测３０例原发性肝癌中ＣＤ４４ｖｍＲＮＡ和ＤＣＣｍＲＮＡ的表达情况，结合其转移相关的临床病理指标分析它们间的关系以及在转移中的作用。结果：３０例受检标本中，ＣＤ４４ｖｍＲＮＡ中度以上表达２３例（７６６７％），ＤＣＣｍＲＮＡ表达缺失者１８例（６０％），且二者基本重合。其中，１３例病人（Ⅰ组，占４３３３％）同时出现ＣＤ４４ｖｍＲＮＡ中度以上表达而ＤＣＣｍＲＮＡ表达缺失，５例ＣＤ４４ｖｍＲＮＡ阴性及微弱表达的病例（Ⅱ组）ＤＣＣｍＲＮＡ为中度以上表达（１６６７％）。肿瘤包膜缺少、门静脉癌栓、瘤周卫星结节形成等与转移有关的病理指标Ⅰ组明显重于Ⅱ组。结论：在原发性肝癌的侵袭转移过程中，ＣＤ４４ｖｍＲＮＡ的高表达与ＤＣＣｍＲＮＡ的表达缺失关系密切，二者间可能有协同关系。ＤＣＣ表达缺失有利于癌细胞从主瘤上脱落而ＣＤ４４ｖ的表达则有利于游离的肿瘤细胞在局部形成转移灶。
Objective: To study the relationship between the expression of metastasis-associated gene CD44v and tumor suppressor gene DCC in primary liver cancer and to observe their role in invasion and metastasis of primary liver cancer. METHODS: RT-PCR was used to detect the expression of CD44v mRNA and DCC mRNA in 30 cases of primary hepatocellular carcinoma. The relationship between them and their role in metastasis were analyzed by combining their clinicopathological parameters related to metastasis. RESULTS: Of the 30 specimens examined, 23 were CD44v mRNA expression (76.67%), and 18 were DCC mRNA expression deficiency (60%). The two were basically coincident. Among them, 13 patients (Group I, 43% and 33%) also had moderately expressed CD44v mRNA but lacked DCC mRNA expression, and 5 cases of CD44v mRNA-negative and weakly expressed (Group II) DCC mRNA expressed moderately or higher (1667) %). The pathological indicators related to metastasis, such as lack of tumor capsule, portal vein tumor thrombus, and formation of nodal nodular satellite nodules, were significantly greater in group I than in group II. Conclusion: During the invasion and metastasis of primary liver cancer, the high expression of CD44v mRNA is closely related to the loss of DCC mRNA expression, and there may be a synergistic relationship between them. Loss of DCC expression favors the detachment of cancer cells from the primary tumor and the expression of CD44v is beneficial to the formation of metastatic tumors by free tumor cells.