我们用间接血凝法对71例流行性脑脊髓膜炎(流脑)患儿进行血凝IgM抗体检测,以观察抗体的出现情况,进行流脑的诊断。现将结果报道如下。材料及方法对象:住院病人71例,有发热、头痛、呕吐;脑脊液细胞数增加,以中性为主,蛋白升高,糖量减低。大部份病人体部出现大小不等的瘀点。少数病人体部无瘀点,但脑脊液或血培养有脑膜炎双球菌生长,故可以确诊为流脑。抗原制备及方法:由于国内主要流行菌为A群脑膜炎双球菌,故制备A群抗原。将自临床标本分离出的A群流脑菌接种于血脂平板,孵育后收集菌落,并 We used indirect hemagglutination method for 71 cases of epidemic meningitis (meningitis) in children with hemagglutination IgM antibody detection to observe the appearance of antibodies for the diagnosis of meningitis. The results are reported below. MATERIALS AND METHODS Subjects: 71 inpatients had fever, headache and vomiting. The number of cerebrospinal fluid cells increased, mainly in the neutral, protein and sugar content decreased. Most patients have different sizes of the body’s petechia. A small number of patients without petechia, but cerebrospinal fluid or blood culture meningococcal growth, it can be diagnosed as meningitis. Antigen preparation and methods: As the major epidemic bacteria in the domestic group A meningococcus, so Group A antigen preparation. Group A meningitidis isolated from clinical specimens was inoculated on lipid plates and colonies were collected after incubation