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逆流分溶法是利用混合物中各组分在不互溶的两液相之间分配系数的不同而达到分离的目的。以前逆流分溶装置是由许多分配管组合而成,操作过程中需要振摇,这样会造成玻璃分配管破碎漏液以及溶剂乳比。70年代国外发展起来两种层析系统。一种叫液滴逆流层析,它的装置由三部分组成:输液部分,分离管部分,检测和收集部分。分离管部分是由内径2mm左右、内壁硅烷化的玻璃管(一般是300～500根)串联而成。先将分离管内充满固定相,然后注入样品,再将流动相连续注入分离管。流动相在固定相中形成一个个小液滴。液滴在固定相中移动摩擦,界面不断更新,使样品中各组分在两相中有效分配。另一种叫螺旋管式逆流层析,它的分离管是由绕成螺旋状的聚四氟乙烯(PTFE)管组成。几根螺旋管串联成一
Countercurrent division is the use of the components of the mixture in the immiscible two liquid phase partition coefficients to achieve the purpose of separation. In the past, the countercurrent dissolving and dissolving device was composed of a plurality of distributing tubes which needed to be shaken during the operation, which would cause the glass distributing tube to break and leak the liquid and the solvent to milk ratio. 70 years abroad developed two kinds of chromatography system. One is called droplet countercurrent chromatography, and its device consists of three parts: the infusion part, the separation tube part, the detection and the collection part. The part of the separation tube is composed of a series of glass tubes (usually 300 to 500) with an inner diameter of about 2 mm and an inner wall of silanization. The separation tube is filled with a stationary phase, then the sample is injected, and then the mobile phase is continuously injected into the separation tube. The mobile phase forms small droplets in the stationary phase. The droplets move in the stationary phase of friction, the interface is constantly updated, so that the components of the sample in the two-phase effective distribution. The other is called spiral tube countercurrent chromatography, and its separation tube is composed of a spirally wound polytetrafluoroethylene (PTFE) tube. A few spiral in series