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染色体核型分析,应按照以下步骤进行: 一、选材:目前国内外均以分裂中期G显带做为常规分析标本.经显微镜初步观察,挑选两张最好的制片进行分析.好的制片要求具备:(1)分裂指数>5,即每低倍视野中分裂细胞不少于5个;(2)染色体分散良好,分裂相呈圆形分布,无丢失或重叠;(3)无胞浆背景,带纹清晰,除深浅带外,能显出灰带及末端浅带;(4)染色体有一定长度.一般要求17号染色体长臂远端深带能分辨为两条(q22及q24);(5)选出的制片应分别取自两个培养瓶.这样当两张制片均发生同样畸变时,可初
Chromosome karyotype analysis, should be carried out in accordance with the following steps: First, the material selection: at home and abroad are split mid G banding as a routine analysis of specimens by microscopic observation, picking the two best producers for analysis. (2) Chromosomes are well distributed and the split phase is circularly distributed with no loss or overlap; (3) Cells without cells (4) Chromosomes have a certain length, and it is generally required that the distal deep zone of chromosome 17 long arm be resolved into two (q22 and q24 ); (5) The selected tablets should be taken from two culture bottles, so that when the same distortion occurs in both tablets,