论文部分内容阅读
目的利用比较蛋白质组学方法获得差异表达蛋白,从中寻找与新疆哈萨克族食管癌发生、发展密切相关的基因,为新疆哈萨克族食管癌的早期诊断和治疗提供依据。方法利用二维凝胶电泳(2-dimensionelectrophoresis,2-DE)和基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF-MS)技术联用检测新疆哈萨克族食管癌癌组织与远端正常组织的差异表达蛋白,对其中表达上调的Atp5a1基因进行RT-PCR验证。结果 2-DE结果显示新疆哈萨克族食管癌癌组织中Atp5a1明显可见,蛋白表达强度差异>1.5倍。RT-PCR验证结果显示,癌组织中Atp5a1mRNA明显升高,差异有统计学意义(P<0.05)。结论 Atp5a1基因的高表达和食管癌的发生、发展密切相关。
OBJECTIVE: To compare differentially expressed proteins by comparative proteomics and to search for genes closely related to the occurrence and development of esophageal cancer in Kazak of Xinjiang, so as to provide evidence for the early diagnosis and treatment of esophageal cancer in Xinjiang Kazakh. Methods Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF-MS) To detect differentially expressed proteins in Kazak esophageal cancer tissues and distant normal tissues in Xinjiang, and to identify the Atp5a1 gene whose expression was up-regulated by RT-PCR. Results The 2-DE results showed that Atp5a1 in Kazakh esophageal cancer tissues was clearly seen> 1.5-fold difference in protein expression intensity. The results of RT-PCR showed that the Atp5a1 mRNA in cancer tissues was significantly increased (P <0.05). Conclusion The high expression of Atp5a1 gene is closely related to the occurrence and development of esophageal cancer.