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抗血清是多克隆性的不均一抗体,单克隆抗体则与其相反,它的化学和免疫学性状是均一的,对一种抗原决定簇有很高的特异性.而且可以长期连续获得同一单克隆抗体(McAb).下面介绍应用McAb设计的新免疫测定技术.一、双侧同时结合法(Simultaneoustwo Site):双侧结合法(夹心法)一般是被检材料(包括被检抗原)和固相抗体及标记抗体的反应分两阶段进行.用针对抗原上不同的抗原决定簇的两种McAb同时与
Antisera are polyclonal, inhomogeneous antibodies, whereas monoclonal antibodies, in contrast, have chemical and immunological properties that are uniform and highly specific to an antigenic determinant, and that the same monoclonal Antibodies (McAb) The following describes the use of McAb design of new immunoassay 1. First, the bilateral simultaneous method (Simultaneousstwo Site): Binding method (sandwich method) is generally the test material (including the test antigen) and the solid phase The reaction of the antibody with the labeled antibody is carried out in two stages with two McAbs directed against different antigenic determinants on the antigen simultaneously with