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本实验用颈动脉血恒速满流毁脑脊髓大鼠(pithed rat)单侧肾脏的方法,观察了高度选择性α_1受体阻断剂哌唑嗪和α_2受体阻断剂育亨宾与去甲肾上腺素、肾上腺素及电刺激肾交感神经对肾血管平滑肌的相互作用。哌唑嗪明显抑制电刺激引起肾灌流压升高,但尚存留一小部分既不能被增加哌唑嗪的剂量所阻断也不能被育亨宾所阻断。由肾动脉直接注射去甲肾上腺素和肾上腺素引起灌流压升高作用的绝大部分可被哌唑嗪所阻断,所存留的一小部分又可被育亨宾所阻断。结果提示,在肾血管平滑肌突触后膜存α_1在和α_2两种受体亚型,以α_1受体为主,但也有很小比例的α_2受体。
In this experiment, we used a method of carotid artery blood flow to destroy the unilateral kidney of the pithed rat. We observed the effects of prazosin and α_2 receptor antagonist yohimbine, a highly selective α_1 receptor antagonist, Norepinephrine, epinephrine and the interaction of renal sympathetic nerves on renal vascular smooth muscle. Prazosin significantly inhibited the electrical stimulation caused by increased renal perfusion pressure, but still a small part of both can not be increased by the dose of prazosin can not be blocked by yohimbine. Most of the effects of noradrenaline and epinephrine on perfusion pressure rise induced by the direct injection of renal arteries can be blocked by prazosin, and a small percentage of the remaining can be blocked by yohimbe. The results suggest that in the vascular smooth muscle postsynaptic membrane survival α_1 and α_2 two receptor subtypes, mainly α_1 receptor, but also a small proportion of α_2 receptors.