原代细胞培养（ｐｒｉｍａｒｙｃｅｌｌｃｕｌｔｕｒｅｓ）：利用胰蛋白酶消化剪成很小的新鲜离体组织块，将它们分散为单个细胞。除去胰蛋白酶后，将分散的细胞悬液与液体培养基一起置于培养容器中；培养液中含有维持等渗浓度所需的离子、多种氨基酸、维生素和葡萄糖，以... Primary cell cultures: Small fresh tissue sections are detached by trypsinization to disperse them into individual cells. After removal of trypsin, the dispersed cell suspension is placed in a culture vessel together with a liquid culture medium containing ions, a variety of amino acids, vitamins and glucose required for maintaining an isotonic concentration, to ...