在杀虫活性追踪指导下,运用萃取、硅胶柱层析、高效液相色谱制备等方法,从杠柳根部甲醇提取物中分离出化合物LY01,经1H NMR1、3C NMR等波谱方法鉴定,LY01为杠柳新甙A(periplocosides A)。生物测定结果表明,LY01为杠柳的主要杀虫活性成分。试虫对样品表现出特殊症状,试虫的中肠及脂肪体被破坏,初步认为LY01是一种消化毒剂。 Under the guidance of insecticidal activity tracking, the compound LY01 was isolated from the methanol extract of Barbarium wilfordii by extraction, silica gel column chromatography and high performance liquid chromatography (HPLC), and identified by 1H NMR1 and 3C NMR spectroscopy. LY01 was Periplocosides A. Bioassay results show that, LY01 is the main insecticidal active ingredient bar. Species showed specific symptoms of test insects, insects midgut and fat body was destroyed, initially considered LY01 is a digestive toxicant.