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设计了一种新的高效筛选程序，从植物根际土壤中分离出一株具有ＡＣＣ脱氨酶活性的促生拮抗双功能假单胞菌株Ｍ１８．在培养皿中，Ｍ１８能有效地抑制黄瓜枯萎病菌以及多种其他枯萎病原菌和植物病原菌的生长．Ｍ１８活菌浸种处理春黄瓜种子以及在大棚定植后根际土壤浇灌的试验中，与对照组相比，黄瓜枯萎病的株发病率降低７０％～８０％，霜霉病的发病率和病情指数都同时平均下降７０％左右，黄瓜产量提高２０％以上，每公顷可增加产值０．９万元以上，Ｍ１８的生物防治效果达到极显著程度．
A new and efficient screening program was designed to isolate a growth-promoting antagonistic bifunctional Pseudomonas strain M18 with ACC deaminase activity from plant rhizosphere soil. In petri dishes, M18 effectively inhibited the growth of Fusarium oxysporum f. Cucurbitacea and many other wilt pathogens and plant pathogens. M18 viable soaking seed treatment of spring cucumber seeds and rhizosphere soils in the greenhouse after planting in the test, compared with the control group, the incidence of cucumber wilt strains reduced by 70% to 80%, downy mildew incidence and disease index At the same time, the average decrease of about 70%, cucumber yield increased by 20% or more per hectare can increase the output value of more than 9,000 yuan, M18 biological control effect reached a significant level.