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目的:通过观察肾衰饮对慢性肾功能衰竭大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达的影响,探讨肾衰饮延缓慢性肾功能衰竭的药效学机制。方法:52只SD大鼠随机分为正常组、模型组、尿毒清组及肾衰饮组。采用腺嘌呤灌胃法制作大鼠肾衰模型。造模成功后肾衰饮组大鼠给予肾衰饮煎剂灌胃,尿毒清组大鼠给予尿毒清颗粒灌胃,各组大鼠连续给药4周,全自动生化分析仪检测血肌酐(SCr)、尿素氮(BUN)及白蛋白水平,HE染色及Masson染色观察各组大鼠肾组织病理变化,Real time-PCR技术检测大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达情况。结果:与正常组比较,模型组血肌酐、尿素氮水平显著升高(P<0.01),白蛋白水平显著降低(P<0.01);与模型组比较,肾衰饮组和尿毒清组大鼠血肌酐、尿素氮水平均显著降低(P<0.01);白蛋白水平均显著升高(P<0.01)。HE及Masson染色显示模型组大鼠肾脏组织发生明显的病理学改变,肾衰饮组及尿毒清组大鼠肾脏病理改变发生明显改善。与正常组相比,模型组大鼠肾脏bmp-7基因mRNA表达水平显著下降(P<0.01),pdgf、timp-1基因mRNA表达水平显著升高(P<0.01);与模型组比较,肾衰饮组大鼠肾脏bmp-7基因mRNA表达水平显著升高(P<0.01),pdgf、timp-1基因mRNA表达水平显著下降(P<0.01)。结论:肾衰饮可能通过调节慢性肾功能衰竭大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达,发挥其延缓慢性肾功能衰竭进展的重要生物学作用。
Objective: To observe the effect of Shenshuai Decoction on the expression of bmp-7, pdgf and timp-1 mRNA in the kidney of rats with chronic renal failure and to explore the pharmacodynamic mechanism of Shenshuai Decoction in delaying chronic renal failure. Methods: Fifty-two Sprague-Dawley rats were randomly divided into normal group, model group, Niaoduqing group and Shenfeiyin group. Rat model of renal failure was made by adenine gavage. After the model was established, the rats in the kidney decoction group were given the decoction of Shenfeiyin decoction, and the rats in the Niaoduqing group were administered with Niaoduqing granule. The rats in each group were continuously administered for 4 weeks. The serum levels of serum creatinine SCr, BUN and albumin. The pathological changes of renal tissue were observed by HE staining and Masson staining. The mRNA expression of bmp-7, pdgf and timp-1 in rat kidney was detected by Real time-PCR. Results: Compared with the normal group, the levels of serum creatinine and urea nitrogen in the model group were significantly increased (P <0.01) and albumin levels were significantly decreased (P <0.01). Compared with the model group, Serum creatinine and urea nitrogen levels were significantly lower (P <0.01); albumin levels were significantly higher (P <0.01). The HE and Masson staining showed that the pathological changes of the kidneys were observed in the model group. The pathological changes of the kidneys in the SHUFA and the Niaoduqing groups were significantly improved. Compared with the normal group, the mRNA expression of bmp-7 in the kidney of the model group was significantly decreased (P <0.01), and the mRNA expression of pdgf and timp-1 in the model group was significantly increased (P <0.01) The expression of bmp-7 gene mRNA in the kidney of the decoction group was significantly increased (P <0.01), and the mRNA expressions of pdgf and timp-1 were significantly decreased (P <0.01). Conclusion: SHR can play an important biological role in retarding the progression of chronic renal failure by regulating the expression of bmp-7, pdgf and timp-1 mRNA in the kidney of chronic renal failure rats.