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目的探讨肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)对大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响。方法用胰酶消化法培养新生Wistar大鼠CFs,加入重组人TWEAK(rhTWEAK)干预48 h,采用四甲基偶氮唑蓝(MTT)法测定细胞增殖,qRT-PCR检测Ⅰ型和Ⅲ型胶原mRNA,Western blot检测Ⅰ型胶原蛋白表达。结果 rhTWEAK作用后CFs的细胞数目明显增加,Ⅰ型和Ⅲ型胶原mRNA表达上调,同时Ⅰ型胶原蛋白表达显著增强。结论 TWEAK具有促进大鼠心肌成纤维细胞增殖和胶原合成的作用。
Objective To investigate the effects of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) on the proliferation and collagen synthesis of rat cardiac fibroblasts (CFs). Methods The CFs of neonatal Wistar rats were cultured with trypsin digestion method. The recombinant human TWEAK (rhTWEAK) was used to intervene for 48 hours. The cell proliferation was measured by MTT assay. The type Ⅰ and type Ⅲ collagen were detected by qRT-PCR mRNA and Western blot were used to detect the expression of type Ⅰ collagen. Results The number of CFs in rhTWEAK group was significantly increased. The mRNA expression of type Ⅰ and type Ⅲ collagen was up-regulated, while the expression of type Ⅰ collagen was significantly increased. Conclusion TWEAK can promote rat cardiac fibroblasts proliferation and collagen synthesis.