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目的研究前列腺素内过氧化物合酶(Ptgs)2基因在BXD重组近交系(RI)小鼠海马组织中的基因表达变化及表达调控。方法使用基因芯片检测BXD RI小鼠海马组织的基因表达。应用遗传基因组学研究方法进行基因表达数量性状基因座(eQTL)定位分析,寻找Ptgs2基因的上游调控位点、下游调控基因以及协同作用的基因。结果 Ptgs2基因在BXD RI小鼠海马组织中特异性高表达,且控制该基因的eQTL为顺式eQTL,该区域同时还是反式eQTL的富集区,偏相关系数分析发现其中有8个基因为Ptgs2基因的下游调控候选基因。皮尔森相关系数分析显示,BXD RI小鼠基因组中有151个基因与Ptgs2密切相关,其中前20个基因与Ptgs2的遗传相关度到达0.59以上。结论遗传基因组学研究能够有效筛选出Ptgs2基因的上游调控位点、下游调节基因及与之高度协同作用的基因,这部分基因是深入研究Ptgs2基因在多种精神类疾病中分子机制的重要靶点。
Objective To study the gene expression and regulation of Ptgs 2 gene in hippocampus of BXD recombinant inbred (RI) mice. Methods Gene chips were used to detect the gene expression in hippocampus of BXD RI mice. Gene locus quantitative trait loci (eQTL) mapping analysis using genetic research methods to find Ptgs2 upstream regulatory sites, downstream regulatory genes and synergistic genes. Results The Ptgs2 gene was highly expressed in the hippocampus of BXD RI mice, and the eQTL of the gene was cis-eQTL, which was also the enrichment region of trans-eQTL. The partial correlation coefficient analysis showed that 8 genes were Ptgs2 gene downstream regulatory candidate gene. Pearson correlation coefficient analysis showed that 151 genes in BXD RI mouse genome are closely related to Ptgs2, of which the genetic correlation between the first 20 genes and Ptgs2 reached above 0.59. Conclusion The study of genetic genomics can effectively screen the Ptgs2 upstream regulatory sites, downstream regulatory genes and their highly synergistic genes, which is an important target for further study on the molecular mechanism of Ptgs2 gene in various psychiatric diseases .