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目的比较两色金鸡菊花不同极性提取物的体外抗氧化活性,并用TLC-Bio技术分析抗氧化活性成分。方法将两色金鸡菊花分别用水、甲醇、乙醇等溶剂提取;采用DPPH自由基以及羟基自由基清除法测定两色金鸡菊花不同极性提取物的自由基清除率,计算C_(50)值,进行比较;建立两色金鸡菊花提取物的薄层展开条件,用薄层生物自显影技术(TLC-Bio)对抗氧化活性最强的提取物进行抗氧化活性成分的指认。结果 DPPH自由基清除法测定结果:水提物C_(50)=0.962 8mg/mL,甲醇提物C_(50)=0.203 0mg/mL,乙醇提物C_(50)=0.031 0mg/mL;羟基自由基清除法测定结果:水提物C_(50)=0.620 9 mg/mL,甲醇提物C_(50)=1.702 3 mg/mL,乙醇提物C_(50)=0.101 1mg/mL;薄层生物自显影技术检测出具有抗氧化活性的斑点有8个,其中指认出3个成分,分别为马里苷、奥卡宁、flavanokanin。结论两色金鸡菊花提取物中,DPPH自由基及羟基自由基清除能力最强的均是乙醇提取物。
OBJECTIVE To compare the antioxidant activity of different polarity extracts of two species of Chrysolophus pictus and analyze the antioxidative components by TLC-Bio technique. Methods Radix Cichorii (Chrysolophus pictorii) were extracted with water, methanol and ethanol respectively. The free radical scavenging rate of different polar extracts of Chrysocolla was determined by DPPH radical scavenging and hydroxyl free radical scavenging. The C 50 value was calculated. (TLC-Bio) was used to identify the antioxidant active components against the strongest antioxidant activity of the extract. Results The results of DPPH radical scavenging assay showed that C 50 = 0.962 8mg / mL, C 50 = 0.203 0mg / mL and C 50 = 0.031 0mg / mL. The results of radical scavenging assay showed that C 50 = 0.620 9 mg / mL, methanol extract C 50 = 1.702 3 mg / mL and ethanol extract C 50 = 0.101 1 mg / mL. There were 8 spots with anti-oxidant activity detected by imaging technique, of which 3 components were identified, namely, marigoldide, okanin, flavanokanin. Conclusion Among the extracts of two colors, the extract of DPPH radical and hydroxyl radical showed the strongest ethanol extract.